The combination of functional data from cytotoxicity and cytokine secretion assays with phenotypic data provides an unprecedented level of understanding of the mode of action of immunotherapy products. New assays in the pipeline include antigen abundance, cell signalling, intracellular staining and molecular profiling
IMMUNOPHENOTYPE
Detect surface markers on individual immune and tumor cells and combine phenotypic data with functional annotations
CYTOTOXICITY ASSAY
Quantify killing effect in bulk and/or count how many single immune cells actively lyse target cells. Add drugs, media or adjuvants to compare potency across different conditions.
SERIAL KILLING ASSAY
Count immune cells that are capable of killing multiple tumor cells. Stratify immune cells based on the number of targets that each immune cells effectively kills.
CYTOKINE SECRETION
Run multiplex analysis of cytokines secreted in bulk or by a single immune cell. Cytokines are captured by microbeads. All washing steps are automated by the robotic system.
IMMUNOPHENOTYPE
Detect surface markers on individual immune and tumor cells and combine phenotypic data with functional annotations
CYTOTOXICITY ASSAY
Quantify killing effect in bulk and/or count how many single immune cells actively lyse target cells. Add drugs, media or adjuvants to compare potency across different conditions.
CYTOKINE SECRETION
Run multiplex analysis of cytokines secreted in bulk or by a single immune cell. Cytokines are captured by microbeads. All washing steps are automated by the robotic system.
SERIAL KILLING ASSAY
Count immune cells that are capable of killing multiple tumor cells. Stratify immune cells based on the number of targets that each immune cells effectively kills.
Accelerating the entire cell therapy development life cycle
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R&D / DISCOVERY
Donor selection for allogeneic therapy, deep analysis of mechanism of action, high-throughput screening of edited cells
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R&D / PRECLINICAL
Immuno-monitoring on live animals, high-throughput safety tests (on-target, off-tumor)
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R&D / CLINICAL
Monitor potency of persistent cells
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GMP / PROCESS DEV.
Side-by-side comparison of process changes
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GMP / MANUFACTURING
Walk-away potency assay for release
Resources
Application note
Characterization of NK cell donors for allogeneic cell therapy through single cell serial killing
Product sheet
Cellply VivaCyte platform
Technical note
A fully automated walkaway sample prep and analysis platform for potency testing
Technical note
Use of AI to automate the identification and characterization of diverse cells within a co-culture array
Technical note
Parallel screening of multiple Effector:Target (E:T) ratios for cell mediated cytotoxicity analysis
Technical note
Long term viability of suspension cells in VivaCyte using a Co-Culture Array microfluidic device with integrated cell perfusion