Many applications, seamless method transfer

The combination of functional data from cytotoxicity and cytokine secretion assays with phenotypic data provides an unprecedented level of understanding of the mode of action of immunotherapy products. New assays in the pipeline include antigen abundance, cell signalling, intracellular staining and molecular profiling

IMMUNOPHENOTYPE
Detect surface markers on individual immune and tumor cells and combine phenotypic data with functional annotations

assay-killing

CYTOTOXICITY ASSAY
Quantify killing effect in bulk and/or count how many single immune cells actively lyse target cells. Add drugs, media or adjuvants to compare potency across different conditions.

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SERIAL KILLING ASSAY
Count immune cells that are capable of killing multiple tumor cells. Stratify immune cells based on the number of targets that each immune cells effectively kills.

CYTOKINE SECRETION
Run multiplex analysis of cytokines secreted in bulk or by a single immune cell. Cytokines are captured by microbeads. All washing steps are automated by the robotic system.

IMMUNOPHENOTYPE

Detect surface markers on individual immune and tumor cells and combine phenotypic data with functional annotations

CYTOTOXICITY ASSAY

Quantify killing effect in bulk and/or count how many single immune cells actively lyse target cells. Add drugs, media or adjuvants to compare potency across different conditions.

CYTOKINE SECRETION

Run multiplex analysis of cytokines secreted in bulk or by a single immune cell. Cytokines are captured by microbeads. All washing steps are automated by the robotic system.

SERIAL KILLING ASSAY

Count immune cells that are capable of killing multiple tumor cells. Stratify immune cells based on the number of targets that each immune cells effectively kills.

Accelerating the entire cell therapy development life cycle

  • R&D / DISCOVERY

    Donor selection for allogeneic therapy, deep analysis of mechanism of action, high-throughput screening of edited cells

  • R&D / PRECLINICAL

    Immuno-monitoring on live animals, high-throughput safety tests (on-target, off-tumor)

  • R&D / CLINICAL

    Monitor potency of persistent cells

  • GMP / PROCESS DEV.

    Side-by-side comparison of process changes

  • GMP / MANUFACTURING

    Walk-away potency assay for release

Resources

Application note

Characterization of NK cell donors for allogeneic cell therapy through single cell serial killing

Product sheet

Cellply VivaCyte platform

Technical note

A fully automated walkaway sample prep and analysis platform for potency testing

Technical note

Use of AI to automate the identification and characterization of diverse cells within a co-culture array

Technical note

Parallel screening of multiple Effector:Target (E:T) ratios for cell mediated cytotoxicity analysis