Applications
Accelerate the development of cancer immunotherapies, at every stage
APPLICATIONS
VivaCyte enables a diverse set of applications throughout the cell therapy development cycle
Discovery
- Comprehensive immune cell characterization
- Deep analysis of mechanism of action
- High-throughput screening of CRISPR-edited cells or TCR-T cells
Preclinical
- Immuno-monitoring of in vivo studies
- Advanced functional
screening of exhaustion
and persistence
Translational
- Immuno-monitoring based on potency of persistent cells
- Correlation of clinical outcome with potency assessment
- Development of biomarkers of enhanced anti-tumor activity
Process Dev
- High throughput process development and optimization
- Rapid and in-depth batch characterization
- Donor validation
Manufacturing
- Walk-away potency assays based on cytotoxicity
- Walk-away potency assays based on cytokine release
- Multiplex analysis of potency and phenotype
Bulk Cytotoxicity Workflows
Automate cell-mediated cytotoxicity assays with a minimum amount of sample
Easily create dose-response curves analyzing multiple Effector : Target ratios identified by the AI-powered software within each channel
Generate an entire dose-response curve per microfluidic channel, up to 16 dose-response curves per CC-Array plate, using only a few thousand effector cells per channel. Bulk cytotoxicity workflows available for suspension target cells.
Multiparametric Workflows
Unravel the heterogeneity of your cell therapy samples
Define potency of each cell subset in your cell therapy batches
Single-cell multiparametric analysis of cytotoxicity and phenotype enables unprecedented depth of analysis:
- Rapidly define frequency of killer and serial killer cells
- Measure potency of cell subsets, e.g. CD4 and CD8, in a single assay using just one microfluidic channel per sample
- Generate a unified potency score, highly reproducible across runs
Combined with parallel screening of multiple conditions in a single run, multiparametric analysis enables comprehensive testing of cell therapy candidates from numerous process options, against multiple target cells, to rapidly identify the most suitable candidates.
Serial Killing Assay
Find your supercharged cells
Quantify frequency of serial killer cells in just 5% of the time taken for conventional bulk assays
A single immune cell can be evaluated for immunophenotype and ability to kill two or more target cells within a time lapsed study. Leveraging this protocol, the CC-Array technology allows quantification of serial killing at a single-cell resolution in just 24 hours instead of weeks needed by conventional bulk assays. The manual tedious work required in re-challenge assays is replaced with a fully automated process requiring just 20 minutes hand-on time.
Unified Potency Score
Comparability through a Unified Potency Score
Streamline your discovery or process development workflows by ensuring comparability among different product variants or process options
A Unified Potency Score (UPS) is calculated from single-cell cytotoxicity data providing a single indicator for the potency of your cells.
Frequency of both killer and serial killer cells contribute to the UPS, for a comprehensive evaluation of the potency of your product.
Once an incubation time and target cell line are defined, the UPS (time, target) is a standardized score allowing direct comparison of multiple products or product candidates, irrespective of the source of your cells, the batch or cell therapy type.
Application notes and Technical Notes
Resources
TN-005_High throughput parallel screening of multiple Effector : Target (E:T) ratios for cell mediated cytotoxicity kinetic analysis using low sample volume
Learn how VivaCyte can generate multiple dose-response analyses in a single CC-Array plate, based on a cell-mediated cytotoxicity assay carried out at multiple E:T ratios
TECHNICAL NOTE | Document number: TN-005
TN-006_A novel method for comparing the potency of CAR-NK cells to support informed donor selection
Introducing a novel Unified Potency Score (UPS) to quantify the potency of cell therapy products with a single indicator, generated by single-cell killing and serial killing data
TECHNICAL NOTE | Document number: TN-006
TN-007_Characterization of NK cell donors for allogeneic cell therapy using single cell serial killing
Learn how a single-cell serial killing assay allows deep characterization of immune cell function and enables ranking of donors for allogeneic NK cell therapy
TECHNICAL NOTE | Document number: TN-007
TN-008_How single-cell multiparametric analysis enables in-depth understanding of functional heterogeneity and killer kinetics in CAR-T cell therapies
Learn how multiparametric analysis allows to define the potency of subpopulations of cells in a single assay through a combined single-cell analysis of cytotoxicity and phenotype carried out on each immune cell